Journal: Kidney International Reports

Article Title: Somatic Mosaicism in a Male Patient With X-linked Alport Syndrome

doi: 10.1016/j.ekir.2019.03.005

Figure Lengend Snippet: Findings of renal biopsy. (a) A glomerulus with segmental sclerosis on periodic acid–Schiff stain. (b) Immunofluorescence staining for alpha 5 (green) and alpha 2 (red) type IV collagen shows segmental absence of glomerular basement membrane staining for alpha 5 type IV collagen. (c, d) Electron microscopy shows glomerular basement membrane (d for high magnification) with irregular thickening, thinning, and lamination of lamina densa, with severe foot process effacement. Bar = 30 μm in (a) and (b); bar = 1 μm in (c).

Article Snippet: Additional immunofluorescence staining for alpha2 and alpha5 type IV collagen (fluorescein isothiocyanate–conjugated monoclonal rat anti-human collagen IV alpha 5 chain and Texas-Red–conjugated monoclonal rat anti-human collagen IV alpha2 chain; SGE-CFT45325; Cosmo Bio Co., Ltd., Tokyo, Japan) was performed with appropriate controls.

Techniques: Staining, Immunofluorescence, Membrane, Electron Microscopy

Journal: Kidney International Reports

Article Title: Somatic Mosaicism in a Male Patient With X-linked Alport Syndrome

doi: 10.1016/j.ekir.2019.03.005

Figure Lengend Snippet: Teaching points

Article Snippet: Additional immunofluorescence staining for alpha2 and alpha5 type IV collagen (fluorescein isothiocyanate–conjugated monoclonal rat anti-human collagen IV alpha 5 chain and Texas-Red–conjugated monoclonal rat anti-human collagen IV alpha2 chain; SGE-CFT45325; Cosmo Bio Co., Ltd., Tokyo, Japan) was performed with appropriate controls.

Techniques: Staining, Next-Generation Sequencing